Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Promotive effect of higenamine on the collagen-related proteins. The levels of TGF- β (a), Smad3 (b), and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗ P < 0.05 and ∗∗ P < 0.01 compared to the control group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on UVB-induced photoaging in the skin of mice. Representative histological analysis of skin section damaged by UVB exposure. (a) Masson's trichrome staining to identify collagen fibers. The levels of Smad3 (b) and COL1A1 (c) determined by ELISA. Values are expressed as the mean ± standard error of the mean. ∗∗ P < 0.01 and ∗∗∗ P < 0.001 compared to the control group, and ## P < 0.01 and ### P < 0.001 compared to the UVB-alone group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: Staining, Enzyme-linked Immunosorbent Assay, Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Effects of the Higenamine, a Potent Compound from Aconitum , on UVB-Induced Photoaging in Hairless Mice

doi: 10.1155/2022/9116642

Figure Lengend Snippet: Effect of higenamine on the levels of UVB-induced photoaging in vitro. Cytotoxicity levels measured by MTT (a), and COL1A1 levels measured by ELISA (b) in cells treated with higenamine followed by UVB stimulation. Levels of Smad2 DNA-binding Phosphorylation (c) and COL1A1 (d) in TGF- β siRNA-transfected differentiated human primary fibroblast cells. Values are expressed as the mean ± standard error of the mean. # P < 0.05 and ### P < 0.001 compared to the control group, ∗ P < 0.05, ∗∗ P < 0.01, and ∗∗∗ P < 0.001 compared to the UVB-alone group, & P < 0.05 compared to the si-TGF- β group, and @ P < 0.05 compared to the higenamine + si-TGF- β + UVB group.

Article Snippet: The COL1A1 ELISA kit was obtained from Cusabio Technology (Wuhan, China).

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Binding Assay, Phospho-proteomics, Transfection, Control

Journal:

Article Title: A mutant, noninhibitory plasminogen activator inhibitor type 1 decreases matrix accumulation in experimental glomerulonephritis

doi: 10.1172/JCI200318038

Figure Lengend Snippet: Immunofluorescent staining score for ECM proteins at d6. Glomerular staining for FN-EDA+ (a), laminin (b), type I collagen (c), and type III collagen (d) were lower in the PAI-1R–treated, nephritic group. *P < 0.001 compared with normal control. #P < 0.01 compared with disease control.

Article Snippet: Monoclonal mouse anti-cellular fibronectin extra domain positive (FN-EDA+) (Harlan Sera-Lab Ltd., Loughborough, United Kingdom), rabbit anti–mouse laminin (ICN Immunobiologicals, Aurora, Ohio, USA), goat anti–human type I collagen, and goat anti–human type III collagen (Southern Biotechnology Associates, Birmingham, Alabama, USA) were used as the primary Ab’s for detection of matrix components in glomeruli at d6.

Techniques: Staining

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Violin plots graphs showing proteins of the epithelial-mesenchymal transition process, matricellular, and the Wnt signaling pathway expression analyzed by QuPath (N=120). The protein expression was demonstrated between three different histologic types in non-small cells lung cancer for (A) E-cadherin, (B) β-catenin, (C) Heparan sulfate, (D) Chondroitin sulfate, (E) Col I, (F) Col III, (G) Col IV, (H) Col V, (I) WNT1, (J) WNT3A, (K) WNT5A, (L) WNT5B, and (M) SPARC. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC, lung squamous cell carcinoma; Col, collagen type.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Co-analysis of immunofluorescence of chondroitin sulfate (green; C , G , K ) and collagen type I (red; B , F , J ) in three different histological subtypes of non-small cell lung carcinoma (N=120). The stained nuclei are represented in blue (DAPI; A , E , I ). Images D , H , L represent the merge of the same field of these three stains. White arrows indicate positive expression of the markers. Original magnification: 40x. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC: lung squamous cell carcinoma.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Co-analysis of immunofluorescence of chondroitin sulfate (green; C , G , K ) and collagen type V (red; B , F , J ) in three different histological subtypes of non-small cell lung carcinoma (N=120). The stained nuclei are represented in blue (DAPI; A , E , I ). Images D , H , L represent the merge of the same field of these three stains. White arrows indicate positive expression of the markers. Original magnification: 40x. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC: lung squamous cell carcinoma.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Co-analysis of immunofluorescence of chondroitin sulfate (green; C , G , K ) and collagen type III (red; B , F , J ) in three different histological subtypes of non-small cell lung carcinoma (N=120). The stained nuclei are represented in blue (DAPI; A , E , I ). Images D , H , L represent the merge of the same field of these three stains. White arrows indicate positive expression of the markers. Original magnification: 40x. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC: lung squamous cell carcinoma.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Co-analysis of immunofluorescence of heparan sulfate (green; C , G , K ) and collagen type IV (red; B , F , J ) in three different histological subtypes of non-small cell lung carcinoma (N=120). The stained nuclei are represented in blue (DAPI; A , E , I ). Images D , H , L represent the merge of the same field of these three stains. White arrows indicate positive expression of the markers. Original magnification: 40x. LCC, large cell carcinoma; ADC, lung adenocarcinoma; SqCC: lung squamous cell carcinoma.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Immunofluorescence, Staining, Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Association between clinicopathologic characteristics and mean expression (% positive expression) of GAGs and collagen types ( t test and ANOVA, P <0.05).

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Expressing

Journal: Frontiers in Oncology

Article Title: Modeling extracellular matrix through histo-molecular gradient in NSCLC for clinical decisions

doi: 10.3389/fonc.2022.1042766

Figure Lengend Snippet: Variables associated with overall survival in 120 non-small cell lung cancer patients.

Article Snippet: The specimens were incubated overnight at 4°C with antibodies against: E-cadherin (1:100; Boster Biological), β-catenin (1:100; Santa Cruz), heparan sulfate (1:500; Santa Cruz), chondroitin sulfate (1:100, Santa Cruz), anti-human collagen type I (1:700; Rockland Inc.), anti-human collagen type III (1:200; Rockland Inc.), anti-human collagen type IV (1:100; Dako), and anti-human collagen type V (1:1000; Rockland Inc.).

Techniques: Expressing